Many of the computer science researchers now work for design automatic system for fast & accurate recognition of tuberculosis(TB), to speed up the treatment process of patient to save patient life. Any type of research is not possible without data. It is critical for computer researcher to prepare Ziehl-Neelsen stain sputum smear slide & to setup image acquisition technique on simple light microscope. We are preparing acid fast stain sputum smear images data set using light microscope withdigital camera at the eye piece of microscope. The dataset contain 400 total images (300 images are positive for Tb and 100 negative for Tb). These captured image dataset is available for many researchers. One of the application is applied on 50 of these images

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Ziehl-neelsen Stain Image Segmentation Acid Fast Stain