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Optimization of Poly h-Hydroxy Butyrate Production by Alcaligenes Latus MTCC2311 using Central Composite Design

by Veeramanikandan. V, Mrudula. S, Yuvaraj. R, Balaji. P
journal cover thumbnail

International Journal of Computer Applications
Foundation of Computer Science (FCS), NY, USA
Volume 68 - Number 25
Year of Publication: 2013
Authors: Veeramanikandan. V, Mrudula. S, Yuvaraj. R, Balaji. P
10.5120/11753-7412

Veeramanikandan. V, Mrudula. S, Yuvaraj. R, Balaji. P . Optimization of Poly h-Hydroxy Butyrate Production by Alcaligenes Latus MTCC2311 using Central Composite Design. International Journal of Computer Applications. 68, 25 ( April 2013), 33-41. DOI=10.5120/11753-7412

@article{ 10.5120/11753-7412,
author = { Veeramanikandan. V, Mrudula. S, Yuvaraj. R, Balaji. P },
title = { Optimization of Poly h-Hydroxy Butyrate Production by Alcaligenes Latus MTCC2311 using Central Composite Design },
journal = { International Journal of Computer Applications },
issue_date = { April 2013 },
volume = { 68 },
number = { 25 },
month = { April },
year = { 2013 },
issn = { 0975-8887 },
pages = { 33-41 },
numpages = {9},
url = { https://ijcaonline.org/archives/volume68/number25/11753-7412/ },
doi = { 10.5120/11753-7412 },
publisher = {Foundation of Computer Science (FCS), NY, USA},
address = {New York, USA}
}
%0 Journal Article
%1 2024-02-06T21:28:53.927381+05:30
%A Veeramanikandan. V
%A Mrudula. S
%A Yuvaraj. R
%A Balaji. P
%T Optimization of Poly h-Hydroxy Butyrate Production by Alcaligenes Latus MTCC2311 using Central Composite Design
%J International Journal of Computer Applications
%@ 0975-8887
%V 68
%N 25
%P 33-41
%D 2013
%I Foundation of Computer Science (FCS), NY, USA
Abstract

Considering the industrial interest of poly-b-hydroxy butyrate (PHB) and its high production cost, work has been undertaken for the production of PHB by Alcaligenes latus (2311). Different industrial wastes (sesame, molasses, sago and paper waste) were used as a cheap substrate to minimize the production of cost and nitrogen limited minimal agar synthetic medium was also used for comparison. Accumulation of PHB granules in the organism was analyzed by sudan black method. The PHB production in various industrial waste based medium and nitrogen limited minimal agar synthetic medium was studied by crotonic acid method. The pure form of PHB was collected and qualitatively analyzed by infrared and nuclear magnetic resonance methods. Highest PHB production was found in nitrogen limited minimal agar synthetic medium. Among the various industrial wastes based media, highest yield was obtained with sesame oil waste as carbon source

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Index Terms

Computer Science
Information Sciences

Keywords

Poly-?-hydroxy butyrate PHB Alcaligenes latus MTCC 2311 CCD bacterial polyesters

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