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Development and Analysis of a Stoichiometric Model of Candidate Bacterium for Bioethanol Production Clostridium Thermocellum
| International Journal of Computer Applications |
| Foundation of Computer Science (FCS), NY, USA |
| Volume 35 - Number 9 |
| Year of Publication: 2011 |
| Authors: Adel Maghsoudpour, Ali Ghaffari, Mohammad Teshnehlab |
10.5120/4426-6163
|
Adel Maghsoudpour, Ali Ghaffari, Mohammad Teshnehlab . Development and Analysis of a Stoichiometric Model of Candidate Bacterium for Bioethanol Production Clostridium Thermocellum. International Journal of Computer Applications. 35, 9 ( December 2011), 1-4. DOI=10.5120/4426-6163
Abstract
In this study we developed a computational model of core metabolism of Clostridium thermocellum which allows for in silico analysis of central metabolic fluxes in this strain. The model was used to predict a number of experimentally observed metabolic phenotypes in C. thermocellum growth cultures including cell growth rate and by-product secretion. Results of the model analysis show a good agreement between experimental data and model predictions. All the reactions included in the model are based on experimental evidence on their actual activity in the metabolism of C. thermocellum. Using flux balance analysis the distribution of flux over central metabolism and fermentative pathways are predicted. The results of model prediction can be further validated when experimental data on actual distribution carbon flux in C. thermocellum becomes available. The developed stoichiometric model can be applied for predicting the consequences of introducing pathways manipulation in this organism with the aim of improving ethanol and hydrogen production yields.
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