We apologize for a recent technical issue with our email system, which temporarily affected account activations. Accounts have now been activated. Authors may proceed with paper submissions. PhDFocusTM
CFP last date
20 November 2024
Reseach Article

Article:Analysis of ZN-Stained Sputum Smear Enhanced Images for Identification of Mycobacterium Tuberculosis Bacilli Cells

by Jadhav Mukti, Kale K.V.
International Journal of Computer Applications
Foundation of Computer Science (FCS), NY, USA
Volume 23 - Number 5
Year of Publication: 2011
Authors: Jadhav Mukti, Kale K.V.
10.5120/2882-3760

Jadhav Mukti, Kale K.V. . Article:Analysis of ZN-Stained Sputum Smear Enhanced Images for Identification of Mycobacterium Tuberculosis Bacilli Cells. International Journal of Computer Applications. 23, 5 ( June 2011), 10-16. DOI=10.5120/2882-3760

@article{ 10.5120/2882-3760,
author = { Jadhav Mukti, Kale K.V. },
title = { Article:Analysis of ZN-Stained Sputum Smear Enhanced Images for Identification of Mycobacterium Tuberculosis Bacilli Cells },
journal = { International Journal of Computer Applications },
issue_date = { June 2011 },
volume = { 23 },
number = { 5 },
month = { June },
year = { 2011 },
issn = { 0975-8887 },
pages = { 10-16 },
numpages = {9},
url = { https://ijcaonline.org/archives/volume23/number5/2885-3760/ },
doi = { 10.5120/2882-3760 },
publisher = {Foundation of Computer Science (FCS), NY, USA},
address = {New York, USA}
}
%0 Journal Article
%1 2024-02-06T20:09:48.463760+05:30
%A Jadhav Mukti
%A Kale K.V.
%T Article:Analysis of ZN-Stained Sputum Smear Enhanced Images for Identification of Mycobacterium Tuberculosis Bacilli Cells
%J International Journal of Computer Applications
%@ 0975-8887
%V 23
%N 5
%P 10-16
%D 2011
%I Foundation of Computer Science (FCS), NY, USA
Abstract

The analysis of ZN-Stain sputum smear images for the proper identification of Mycobacterium tuberculosis. For controlling on TB the early detection of TB is important this detection of Tb through microscope, in low- and middle-income countries. ZN-Staining commonly used for detecting M.TB bacilli from sputum. Tuberculosis (TB) diagnosis by manual observation varies depending on the quality of the smear and skill of the pathologist .This manual screening method is time consuming, tedious & sometime it may confusing with some Non tuberculosis bacilli or some rod shape stain residue. Due to this we gets the faulty results. For this reason need of atomization is required. We present an enhancement method on images of Ziehl-Nelson stained sputum smears obtained using a bright field microscope. Some time the original images are noisy, degrade or blurred it need to preprocessing, enhancing the image for better result. Many of the scientist worked on gray level images or some used color images. We compare contrast stretching (High contrast) technique with sharping linear spatial filter by unsharp masking for improving the quality of ZN-Stained Sputum Smear image. The contrast stretching gives better results than the sharping image by linear spatial unsharp masking.

References
  1. WHO Report 2009,“Global tuberculosis Control a short update to the 2009 report”.
  2. Module for MPWs and other DOT providers. Revised National Tuberculosis Control Program, Directorate general health and family welfare, Nirman bhavan New Delhi March 2006.
  3. J. A. C. Luna, “A Tuberculosis Guide for Specialist Physicians”, International Union against Tuberculosis and Lung Disease,2004.
  4. K. Veropoulos, G. Learmonth, C. Campbell, B. Knight, and J. Simpson, “Automatic identification of tuberclebacilli in sputum. a preliminary investigation,” Analytical and quantitative cytology and histology 21(4), pp. 277-281, 1999.
  5. K. Veropoulos, C. Campbell, G. Learmonth, B. Knight, and J. Simpson, “The automatic identification of tubercle bacilli using image processing and neural computing techniques,” in Proceeding of the 8th international conference on artificial neural networks, 2, p. 797, 1998.
  6. M. Wilkinson, Fluorescence microscopy and fluorecent probes, ch. Rapid automatic segmentation of fluorescent and phase-contrast images of bacteria.
  7. J. Alvarez-Borrego, R. Mourino,G. Cristobal, and J. Pech, “Invariant optical color correlation for recognition of vibrio choleraeo1,” in Int. conf. on pattern recognition, 2847, p. 283, (Barcelona, Spain), 2000.
  8. P. Demantova, D. Sakamoto, S. Ioshii, and H. Gamba, “Segmentacao autom´atica de bact´erias para o m´etodo deft,” in Proceedings of the II latin american congress on biomedical engineering, (Havana, Cuba), 2001.
  9. M. Forero, E. Sierra, J. Alvarez-Borrego, J. Pech, G. Crist´obal, L. Alcal´a, and M. Desco, “Automatic sputum color segmentation for tuberculosis diagnosis,” in Algorithms and systems for optical information processing, 2001.
  10. M. Forero, F. Sroubek, J. Alvarez-Borrego, N. Malpica, G. Crist´obal, A. Santos, L. Alcal´a, M. Desco,and L. Cohen, “Segmentation, autofocusing and signature extraction of tuberculosis sputum images,” in Photonic devices and algorithms for computing, 2002.
  11. Manuel G. Forero, Gabriel Cristobal and Josue Alvarez-Borrego “Automatic identification techniques of tuberculosis bacteria”
  12. R.A.A.Raof, ZalehaSalleh, S.I. Sahidan,M.Y. Mashor et,al “Color Thresholding Method For Image Segmentation Algorithm Of Ziehl-Neelsen Sputum Slide Images” 2008
  13. Vishnu Makkapati, Ravindra Agrawal and Raviraja Acharya “Segmentation and Classification of Tuberculosis Bacilli from ZN-stained”, 5th Annual IEEE Conference on Automation Science and Engineering Bangalore, India, August 22-25, 2009
  14. Tuberculosis Training Module, Govt of Maharashrta.
  15. R. C. Gonzalez, and R. E. Woods, Digital Image Processing, Third Edition. Pearson Education, 3rd Ed., Copyright 2008.
  16. R. Fisher, S. Perkins, A. Walker, E. Wolfart (2003), "Contrast Stretching", http ://homepages.inf.ed.ac.uk/rbf/HIPR2/stretch.htm
  17. R. Fisher, S. Perkins, A. Walker, E. Wolfart (2003), "Unsharp filter", http://homepages.inf.ed.ac.uk/rbf/HIPR2/unsharp.htm
  18. Smail Avcıbas, Bu¨ lent Sankur, Khalid Sayood “Statistical evaluation of image quality measures”, 206 / Journal of Electronic Imaging / April 2002 / Vol. 11(2)
  19. Image Statistics Based on material from Digital Imaging: Theory and Applications, H. E. Burdick, McGraw-Hill, 1997)
Index Terms

Computer Science
Information Sciences

Keywords

Enhanced images Mycobacterium Tuberculosis (M.TB) Acid Fast Bacilli (AFB) Contrast stretching ZN-Stained (Ziehl –Neelsen) Sharpening image