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In-Silico Design of a Potent siRNA Molecule for Gene Silencing in Zaire Ebolavirus

by Gopaluni Sai Akash, Jayasree Ganugapati
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International Journal of Computer Applications
Foundation of Computer Science (FCS), NY, USA
Volume 113 - Number 4
Year of Publication: 2015
Authors: Gopaluni Sai Akash, Jayasree Ganugapati
10.5120/19815-1627

Gopaluni Sai Akash, Jayasree Ganugapati . In-Silico Design of a Potent siRNA Molecule for Gene Silencing in Zaire Ebolavirus. International Journal of Computer Applications. 113, 4 ( March 2015), 23-27. DOI=10.5120/19815-1627

@article{ 10.5120/19815-1627,
author = { Gopaluni Sai Akash, Jayasree Ganugapati },
title = { In-Silico Design of a Potent siRNA Molecule for Gene Silencing in Zaire Ebolavirus },
journal = { International Journal of Computer Applications },
issue_date = { March 2015 },
volume = { 113 },
number = { 4 },
month = { March },
year = { 2015 },
issn = { 0975-8887 },
pages = { 23-27 },
numpages = {9},
url = { https://ijcaonline.org/archives/volume113/number4/19815-1627/ },
doi = { 10.5120/19815-1627 },
publisher = {Foundation of Computer Science (FCS), NY, USA},
address = {New York, USA}
}
%0 Journal Article
%1 2024-02-06T22:50:06.558107+05:30
%A Gopaluni Sai Akash
%A Jayasree Ganugapati
%T In-Silico Design of a Potent siRNA Molecule for Gene Silencing in Zaire Ebolavirus
%J International Journal of Computer Applications
%@ 0975-8887
%V 113
%N 4
%P 23-27
%D 2015
%I Foundation of Computer Science (FCS), NY, USA
Abstract

Zaire ebolavirus (EBOV) is one of the most dangerous and the unknown to humans. This is a filamentous virus of the family Filoviridae. Genetic studies of EBOV have shown that it has a negative-stranded RNA as genetic material and seven genes in its genome. One among these genes is the gene 'L', that code for a protein L (Large structural protein), this protein functions as an RNA dependent RNA polymerase. RNAi (RNA interference) is an influential method for post-transcriptional gene slicing in a specific sequence. This is done with the help of a dsRNA called siRNA. Slicing a target mRNA would mean that the mRNA will not be able to produce any protein and the viral activity can be restrained. This mechanism is observed naturally in the organism to regulate the protein production and also as a defense mechanism against some viruses. In-silico construction of siRNA is possible using computational methods in biology. Construction of siRNA is guided by many parameters and the efficiency of the cleavage of mRNA with siRNA is determined by hybridization thermodynamics. The constructed siRNA is potent of knocking down the activity of the virus. This can lead to the discovery of an effective antiviral drug against EBOV.

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Index Terms

Computer Science
Information Sciences

Keywords

RNAi siRNA EBOV Zaire Ebola

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